Jun 27, 1970 · Gene cloning and PCR allow scientists to make a large amount of DNA from only a small fragment. How do these technologies work?

Dec 4, 2025 · Cloning is a method that is used to produce genetically identical copies of pieces of DNA, cells or organisms. Cloning methods include: molecular cloning, which makes copies of …

recombinant DNA technology / DNA cloning; gene cloning; cloning A technology that uses enzymes to cut and paste together DNA sequences of interest.

The cloning of foreign DNA in Escherichia coli episomes is a cornerstone of molecular biology. The pioneering work in the early 1970s, using DNA ligases to paste DNA into episomal …

Sep 16, 2024 · This study introduces a novel cost-effective technique for cloning of linear DNA plasmid inserts, aiming to address the associated expenses linked with popular in vitro DNA …

Jun 17, 2025 · A robust in vivo method for engineering plasmid DNA in E. coli using a triple-selection system that works at any copy number, enabling fast, scalable construction of …

Jan 23, 2023 · To overcome these limitations and start writing DNA as effectively as it is read, alternative technologies have been developed including molecular assembly and cloning …

Prior to the development of PCR in the 1980s, the primary method for producing many copies of a gene was a relatively time-consuming process known as DNA cloning.

Jun 6, 2016 · All cloning procedures (insertions, deletions, mutagenesis and sub-cloning) can be performed and combined at will, using a single universal protocol consisting exclusively of a …

Apr 12, 2009 · The combination of 5′ exonuclease, DNA polymerase and ligase with overlapping DNA fragments facilitates the in vitro assembly of large DNA constructs, including an entire …