Cells are homogenized in guanidinium thiocyanate and the RNA is purified from the lysate by extraction with phenol-chloroform ... nm of an aliquot of the final preparation. This protocol was ...

Compounds with a phenol group will form a blue, violet, purple, green, or red-brown color upon addition of aqueous ferric chloride. This reaction can be used as a test for phenol groups. Mix several ...