Adenosine diphosphate (ADP) or adenosine triphosphate (ATP)-containing important biological molecules can modify macromolecules like proteins and nucleic acids to alter their function in the cell.

We constructed a fluorescent sensor of adenylate nucleotides by combining a circularly permuted variant of GFP with a bacterial regulatory protein, GlnK1, from Methanococcus jannaschii. The sensor's ...

ATP, the principal energy currency of the cell, fuels most biosynthetic reactions in the cytoplasm by its hydrolysis into ADP and inorganic phosphate. Because resynthesis of ATP occurs in the ...

Researchers used a novel phosphate-activation reagent, 2-MeImIm-Cl, in an improved coupling reaction to increase the yield of ADP- or ATP-containing molecules. Unlike traditional reactions, the ...